ABSTRACT
Objective: to compare the healing process of pressure ulcers treated with cryopreserved human amniotic membrane allograft and routine pressure ulcer care in our hospital
Methods: from January 2012 to December 2013, in a prospective randomized clinical trial [IRCT201612041335N2], 24 patients with second and third stage of pressure ulcers were enrolled in this study. All patients needed split thickness skin grafts for pressure ulcer-wound coverage. Selected patients had symmetric ulcers on both upper and lower extremities. The patients were randomly divided into two groups: amnion and control. In the amnion group, the ulcer was covered with cryopreserved amniotic membrane and in the control group it was treated with local Dilantin powder application. The duration and success rate of complete healing was compared between the two groups
Results: the study group was composed of 24 pressure ulcers in 24 patients [19 males and 5 females] with a mean age of 44+/-12.70 years. The demographic characteristics, ulcer area, and underlying diseases were similar in both groups. The early sign of response, such as decrease in wound discharge, was detected 12-14 days after biological dressing. Complete pressure ulcer healing occurred only in the amnion group [p<0.001]. Partial healing was significantly higher in the amnion group [p<0.03]. Healing time in this group was faster than that the control group [20 days versus 54 days]. No major complication was recorded with amniotic membrane dressing
Conclusion: cryopreserved amniotic membrane is an effective biologic dressing that promotes reepithelialization in pressure ulcers
ABSTRACT
Objective: Mesenchymal stromal cells [MSCs] are considered as an excellent source in regenerative medicine, but availability and ethical problems limited their routine use. Therefore, another available source with easy procedure and exempt from ethical debate is important. The purpose of this study is to isolate and characterize the MSCs from human placenta
The stromal cells were isolated from Placental Decidua Basalis [PDB-MSC], Umbilical cord Wharton's Jelly [WJ-MSC] and Amniotic Membrane [AM-MSC]
Methods: Full term human placentas [n=4], from cesarean section delivery were collected. Small fragments from different parts were cultures as explants
The immunophenotyping, mesodermal differentiation, growth kinetics and sternness gene expression was studied
Results: The cultivated cells from three sources expressed CD44, CD105, and CD90. Gene expression of NANOG and OCT4 confirmed the undifferentiated state. The doubling-times for WJ-MSCs, PLC-MSCs and AM-MSCs, respectively, were 21+/-8h, 28+/-9h and 25+/-9h at passage three and 30+/-5h, 45+/-7h and 45+/-7h at passage tenth
The proliferative potential of WJ-MSCs tended to be higher than the other two sources
Conclusion: The fetal derives stromal cells; especially the early passages of WJ-MSCs are available supplies for large scale production of MSC for using in clinical studies or research projects
ABSTRACT
The aim of this study was to examine the effect of crude bile on the human HepG2 and CCRF-CEM cell lines. Cells were exposed to different dilutions of bile. Antiproliferative effects were determined by the cytotoxic MTT assay. Cells undergoing apoptosis were identified by terminal deoxynucleotidyl transferase dUTP nick end labeling [TUNEL] assay. Bile administration resulted in dose-dependent cytotoxicity in both HepG2 and CCRF-CEM cell lines. Incubated cells exhibited morphologic features of apoptosis. Bile has significant cytotoxic activity in HepG2 and CCRF-CEM cancer cells via induction of apoptosis. The mechanism of apoptosis needs to be further evaluated. It may have clinical utility in the treatment of cancer after in vivo confirmation of activity